Plant Pest Containment Level 2 Certification Checklist
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The information provided in this checklist will help the Office of Biohazard Containment and Safety evaluate the physical and operational components of your facility as described in the Containment Standards for Facilities Handling Plant Pests. Biosafety Specialists will review the completed checklist to determine if your facility meets the Plant Pest Containment level 2 requirements (PPC-2).
- Postal Address:
Room(s) / Building(s):
Type of Facility:
Box Greenhouse Box Laboratory Box Other
1) Facility Supervisor
- Phone number:
- Fax number:
- Language preference: Box English / Box Français
2) Biosafety Officer (or equivalent)
- Phone number:
- Fax number:
- Language preference: Box English / Box Français
The information you provide on this document is collected by (for) the Canadian Food Inspection Agency under the authority of Section 34 of the Plant Protection Regulations for the purpose of issuing a permit to import. Information may be accessible or protected as required under the provisions of the Access to Information Act.
Physical and Operational Components
The following tables describe physical and operational containment requirements for Plant Pest Containment (PPC) Level 2 facilities (e.g. laboratories, greenhouses) working with plant pests.
The following symbols are used:
Note: Recommended items are optional, depending upon the nature of the pest(s) requiring containment.
|1||22.214.171.124||Is appropriate security provided for the building (e.g. fencing, motion sensors, physical barriers, patrols)?||Box||Box|
|2||126.96.36.199||Is signage installed on entry doors within the containment zone indicating containment level, contact information, and entry requirements?||Box||Box|
|3||188.8.131.52||Is entrance to the containment zone via self-closing and lockable doors?||Box||Box|
|4||184.108.40.206||Is restricted access to the containment zone ensured through a controlled access system (e.g. electronic access card, code or equivalent)?||Box||Box|
|5||220.127.116.11||Is entrance and exit to the containment zone via an anteroom or a corridor?||Box||Box||Specify:
|6||18.104.22.168||Are anteroom doors self-closing and never opened simultaneously (interlocking doors, and audible or visual alarms are acceptable)?||Box||Box|
|7||22.214.171.124a||Can emergency exits be opened only from inside the containment zone?||Box||Box|
|8||126.96.36.199b||Are emergency exits alarmed?||Box||Box|
|9||188.8.131.52c||Are emergency exits identified as "Emergency Exit Only"?||Box||Box|
|10||184.108.40.206||Are dedicated laboratory clothing and personal protective equipment stored separately from street clothing?||Box||Box|
|11||220.127.116.11||In wet areas, are floors slip-resistant?||Box||Box|
|12||18.104.22.168||Are interior coatings easy to clean and resistant to gas and chemicals, as well as to repeated disinfection in accordance with function (e.g. will withstand disinfection, fumigation)?||Box||Box|
|13||22.214.171.124a||Are bench tops non-absorptive, impervious to water, and resistant to acids, alkalis, organic solvents and moderate heat?||Box||Box|
|14||126.96.36.199b||Are backsplashes installed tight to wall and sealed at wall-bench junction?||Box||Box|
|15||188.8.131.52||Is an autoclave or other validated means of waste treatment/disposal located within the containment zone, or if not available in the containment zone, are procedures in place to safely transport waste for treatment/disposal?||Box||Box||Specify:
Box within PPC-2 zone
Box outside PPC-2 zone
Method of waste treatment (include time and temperature parameters):
|16||184.108.40.206||Is the autoclave equipped with a cycle log recorder to record time, temperature, and pressure?||Box||Box|
|17||220.127.116.11||Is the containment zone screened or sealed (including all windows, supply/exhaust air ducts)?||Box||Box|
|18||18.104.22.168||Is a handwashing sink located within the containment zone and near the point of exit?||Box||Box|
|19||22.214.171.124||Is the handwashing sink provided with "hands-free" capability?||Box||Box|
|20||126.96.36.199||Are appropriate primary containment devices available (e.g. BSCs) to minimize the potential contamination of the containment zone?||Box||Box|
|21||188.8.131.52||Are emergency eyewash facilities provided in the containment zone in accordance with activities and applicable regulations (i.e. ANSI Z358.1-2004)?||Box||Box|
|22||184.108.40.206||Is an emergency shower provided in the containment zone in accordance with activities and applicable regulations (i.e. ANSI Z358.1-2004)?||Box||Box|
|23||220.127.116.11||Are facility service supply controls located both inside and outside of the containment zone to facilitate servicing?||Box||Box|
|24||18.104.22.168||Are soil traps installed in drains?||Box||Box|
|25||22.214.171.124||Is the laboratory adequately equipped (e.g. BSCs, thermocyclers, ELISA plate readers, centrifuges and microscopes) to avoid moving equipment into or out of the containment zone?||Box||Box|
|26||126.96.36.199||Is an alarm system to be installed to detect loss of containment due to unauthorized entry or mechanical or power failure?||Box||Box|
|27||188.8.131.52||Are monitoring and security systems installed to monitor critical containment systems?||Box||Box|
|28||184.108.40.206||Is emergency power available for HVAC, lighting, BSCs, essential equipment and other safety systems?||Box||Box|
|29||220.127.116.11||Is vacuum pump contamination minimized by filtration of vacuum line and use of disinfectant traps?||Box||Box|
|30||18.104.22.168||Are greenhouses designed to withstand extremes of local weather and anticipated maximum snow and ice loads, as well as wind, windborne debris and hail?||Box||Box|
|31||22.214.171.124a||Are greenhouses constructed with a rigid reinforced frame with walls, floors and glazing to form a shell?||Box||Box|
|32||126.96.36.199b||Are all perforations and joints in greenhouses and between the greenhouse and other contained structures sealed to provide a continuous containment barrier?||Box||Box|
|33||188.8.131.52||Are greenhouse floors impervious to water and easy to clean (e.g. concrete)?||Box||Box|
|34||184.108.40.206||Are spare greenhouse window panels, emergency glazing and screening stored nearby for emergency repairs?||Box||Box|
|35||220.127.116.11||Are greenhouse glazings sealed to the greenhouse framework with a sealant that provides a tight, flexible and continuous seal resistant to degradation by chemical disinfectants, UV radiation and temperature changes?||Box||Box|
|36||18.104.22.168||Are greenhouse vents and greenhouse HVAC penetrations screened with appropriate mesh screening to prevent pest escape?||Box||Box|
|37||22.214.171.124||Is the greenhouse ventilation system designed to allow for greenhouse fumigation and pesticide applications?||Box||Box|
|38||126.96.36.199||Are greenhouse personnel who apply pesticides appropriately trained and protected?||Box||Box|
|39||188.8.131.52||Are areas surrounding greenhouses kept free of debris, garbage, compost piles and overhanging shrubs and trees?||Box||Box|
|40||4.1.1||Is access to containment zone and support areas restricted to authorized personnel only?||Box||Box|
|41||184.108.40.206||Is an up-to-date inventory of all imported plant material and plant pests maintained?||Box||Box|
|42||220.127.116.11a||Is a Procedures Manual covering safety and general laboratory and greenhouse operations, including entry and exit protocols and cleaning schedules available to all staff?
Note: if no, proceed to Q#44.
|43||18.104.22.168b||Is the Procedures Manual reviewed and updated regularly?||Box||Box|
|44||22.214.171.124||Is an Emergency Response Plan available that describes emergency procedures, including those for accidents, fires, chemical spills, air handling failure, BSC failure, power loss and containment loss?
Note: plan must cover emergency entry/exit procedures, corrective actions and notification of key personnel and government officials.
|45||126.96.36.199||Are records of activities in the facility kept for three years, including records of all building and equipment maintenance, shipments received, confirmations of pest identification, dates of import, CFIA Permits to Import, associated imported plant material, associated organisms detected, decontamination of packaging materials and transfer of plant pests or organisms to other facilities where authorized by a CFIA inspector?
Note: records shall also be kept of all inoculations or infestations of plant material and the movement of plant material and plant pests into or out of containment.
|46||4.1.3a||Is personnel provided with training on pest-associated hazards and the precautions necessary to prevent the release of contained pests?||Box||Box|
|47||4.1.3b||Is personnel required to show evidence that they know and understand the required precautions?||Box||Box|
|48||4.1.3c||Is the training documented?||Box||Box|
|49||4.1.3d||Are refresher and retraining programs implemented?||Box||Box|
|50||188.8.131.52a||Is personnel working in the containment zone trained in, and follow the Standard Operating Procedures for the area?||Box||Box|
|51||184.108.40.206b||Are trainees supervised by a trained staff member?||Box||Box|
|52||220.127.116.11c||Are visitors, maintenance staff, janitorial staff and others provided with training and/or supervision commensurate with their anticipated activities in the containment zone?||Box||Box|
|53||18.104.22.168a||Are personnel and visitors entering the containment zone required to wear protective clothing up to and including full coverage protective clothing?||Box||Box|
|54||22.214.171.124b||Is protective clothing removed prior to exiting the containment zone?||Box||Box|
|55||126.96.36.199a||Are dedicated or disposable footwear (e.g. rubber boots, shoe covers) worn when working with soil or soilborne pests in situations where the floor may be contaminated with infested plant material or soil?||Box||Box|
|56||188.8.131.52b||Is dedicated or disposable footwear removed for reuse or decontamination prior to exiting the containment zone?||Box||Box|
|57||184.108.40.206||Are BSCs or other primary containment devices used for procedures that involve high concentrations or large volumes of plant pests or their propagules?||Box||Box|
|58||220.127.116.11||Are all organisms rendered non-viable prior to disposal?||Box||Box||Specify method:|
|59||18.104.22.168||Are good laboratory practices employed to prevent the escape of pests?||Box||Box|
|60||22.214.171.124||Are doors kept closed to reduce the potential movement of plant pests?||Box||Box|
|61||126.96.36.199||Is eating, chewing gum, drinking, smoking, storing of food and utensils, storing of personal belongings, applying cosmetics, and inserting or removing contact lenses prohibited in the containment zone?
Note: the wearing of contact lenses is recommended only when other forms of corrective eyewear are not suitable.
|62||188.8.131.52||Is long hair tied back or restrained so that it cannot come into contact with hands, specimens, containers or equipment?||Box||Box|
|63||184.108.40.206||Are all pests and materials in a containment zone treated in accordance with the highest containment requirement for that area (e.g. if PPC-1 and PPC-2 pests are in the same room, PPC-2 practices must be followed)?||Box||Box|
|64||220.127.116.11||Are all pests and material infested or suspected of being infested with a pest moved or transported in containers that are secure, leak-proof and not easily broken?
Note: the containers may only be opened within a facility that provides the appropriate containment level for the pest in question.
|65||18.104.22.168||Are all work areas within a containment zone, including dedicated clerical work stations, clean and tidy?
Note: storage of materials should be minimized, and paperwork should be done outside of containment zones if this presents a risk of disseminating pests.
|66||22.214.171.124||Are cultures stored in sealed, preferably break-resistant, containers such as screw-top vials?
Note: cultures are to be clearly identified and dated. Where possible, petri dish cultures of sporulating fungi should be sealed with stretch film.
|67||126.96.36.199||Are contaminated materials and equipment properly cleaned and decontaminated before leaving the facility for servicing or disposal?||Box||Box||Specify method of decontamination:|
|68||188.8.131.52||Are all unintentionally introduced pests rendered non-viable, including those contaminating cultures, as soon as they are detected?||Box||Box|
|69||184.108.40.206||Are disinfectants effective against the organisms in use available at all times when plant pests are handled or stored?||Box||Box||Specify disinfectant used and contact time:|
|70||220.127.116.11||Are sanitation practices implemented when working with plants and plant pests?
Note: refer to page 37 of the Containment Standards for Facilities Handling Plant Pests for more details.
|71||18.104.22.168||Are autoclaves used for decontamination monitored regularly using biological indicators to ensure efficacy (e.g. consider weekly or monthly monitoring, depending on the frequency of use of the autoclave).
Note: monitoring records must be kept for three years.
|72||22.214.171.124a||Are losses of containment reported immediately to the laboratory supervisor and remedied as soon as possible?||Box||Box|
|73||126.96.36.199b||Are written reports of such incidents maintained for three years, and the results of incident investigations used for continuing education?||Box||Box|
|74||188.8.131.52||Is an effective bird, rodent, weed and plant pest control program maintained to prevent entry and eliminate undesired pests from the containment zone?||Box||Box|
|75||184.108.40.206||Are personnel instructed not to bring personal belongings (e.g. hats, coats, purses) into the containment zone?||Box||Box|
|76||220.127.116.11||Is a basic tool kit available inside the containment zone?||Box||Box|
|77||18.104.22.168||Are all packages of pests from foreign sources opened in a BSC or a sleeved cage, and packaging material decontaminated as soon as possible?||Box||Box|
|78||22.214.171.124||Are footbaths (e.g. trays containing cloth pads soaked in disinfectant) provided in the anteroom of facilities containing soilborne pests, to disinfect footwear, shoe covers or dedicated footwear?||Box||Box|
|79||126.96.36.199||Is protective clothing decontaminated (e.g. heat-treated, frozen, autoclaved or soaked in a 5% bleach solution) before laundering?
Note: clothing does not need to be decontaminated if there are laundering facilities within the containment zone and the facilities have been proven to be effective in killing the pests in use.
|80||188.8.131.52||If there is a risk of disseminating pests with the movement of paper, is an electronic communication system used to transfer information and data from the containment zone?||Box||Box|
|81||184.108.40.206||Are all liquids potentially contaminated by pests decontaminated?
Note: liquids must be collected and treated with steam, heat, chemicals or other proven and validated treatment technology prior to discharge into sewer or septic systems.
|82||220.127.116.11||Are periodic inspections of the containment zone made by facility staff to check for faults and deterioration (e.g. deteriorated door seals and brushes, screens or caulking)?
Note: corrective action must be taken and records kept for three years. Such inspections shall occur at least every six months
|83||18.104.22.168||Are supply and exhaust filters, pre-filters and screens inspected and cleaned or replaced by a designated person on a regular basis?
Note: if no filters are installed, proceed to Q#84.
|84||22.214.171.124||Is inward directional airflow confirmed on a regular basis using a smoke pencil, tape, tissue or other suitable means?
Note: if no inward directional airflow, proceed to Q#85.
|85||126.96.36.199||Are all plant material and insect traps inspected on a regular basis?
Note: all debris and dead plant material should be removed so that it does not act as a refuge for plant pests.
Internal Use Only
Reviewed by: Date:
Certified as: Box PPC-2 Greenhouse Box PPC-2 Laboratory Box PPC-2 Other
Certification due date:
Box Letter faxed/mailed to facility
- Date modified: